- Evaluation of DNA Ploidy and Other Morphometric Parameters of Ovarian Mucinous Tumors.
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Yun Mee Kim, Sang Woo Juhng, Joo Yong Yoo, Kyu Hyuk Cho
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Korean J Pathol. 1991;25(5):397-406.
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- Biological behavior of malignant tumors has been assessed by morphological grading, clinical staging, and estimating other tumor markers. Recently DNA ploidy measured by flow cytometry and image analyser has been suggested as an additional useful indicator of the tumor behavior. In order to extract useful tumor cell-specific information in ovarian mucinous tumors, DNA contents and other morphologic parameters were measured by image analysis and DNA ploidy was also measured by flow cytometry. In all cases of cystadenoma, DNA diploidies were observed. In borderline malignancy, DNA diploidies were chiefly observed except one case of polyploidy. In true malignancy, DNA aneuploidies were observed except one case of polyploidy and two cases of diploidies by image analysis, and except four cases of diploides and one cas of polyploidy by flow cytometry. The statistical significance were observed in DNA ploidy pattern by image analysis. In nuclear areas, perimeters and major axis, statistical significance were not observed. These results suggest that DNA ploidy pattern are more or less independent parameter as an additional useful indicator of the histological grade of malignancy and that image analysis are better than flow cytometry in detecting DNA aneuploidy.
- Cytopathologic Observation of Primary Malignant Melanoma of the Lung: A case report.
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Yun Mee Kim, Jong Hee Nam, Min Cheol Lee, Joo Yong Yoo, Kyu Hyuk Cho
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Korean J Pathol. 1991;25(4):367-375.
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- The pulmonary cytology has reached a high level of accuracy.
By the examination of the sputum and/or bronchial brushings, it is now possible to make a diagnosis in 70% to 90% of patients with cancer. Primary melanoma of the lung is very rare and there have been reported about 20 cases in the world literature. We present a case of primary malignant melanoma of the lung in a 61-year-old male diagnosed by cytologic examination of sputum, bronchial brushing and aspirated pleural fluid. Histologic examination of bronchoscopic biopsy and examination of the skin and other primary sites confirmed the diagnosis.
- Evaluation of DNA Ploidy of Bronchogenic Carcinomas by Image Analysis.
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Soo Sung Kim, Jae Hyuck Lee, Sang Woo Jung, Joo Yong Yoo
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Korean J Pathol. 1991;25(3):238-244.
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- In order to extract useful tumor cell-specific information.
DNA contents and other morphological parameters were measured by image analysis. Single cell preparation was made from archived paraffin blocks of 14 cases of bronchogenic squamous cell carcinoma, poorly differentiated, by protease treatment. The cells were Feulgen stained, and DNA content, area, perimeter, and major axis of the tumor cell nuclei were measured. Inflammatory lymphocytes concurrent with the tumor cells were used as an internal standard. DNA ploidies of the lymphocytes and 2C tumor cells showed simple peaks with Gaussian distribution and mean coefficients of variation of 10% and 14% respectively. By the location and proportion of the tumor cells other than 2C cells, DNA ploidies could be classified into diploidy(1 case), polyploidy(2 cases), and aneuploidy(11 cases). The mean proportion of DNA aneuploidal tumor cells relative to the total tumor cells was 82.8%. In 8 cases, nuclear areas showed more or less overlapped distribution, whereas DNA contents showed discrete peaks. THes results suggest that many bronchogenic squamous cell carcinomas, poorly differentiated, have DNA aneuploidy and high proportion of aneuploidal cells, and that nuclear size and DNA content are more or less independent parameters.
- Immunohistochemical Study on the Ha-ras p21 Expression in the Gastric Carcinoma.
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Kwang Min Lee, Joo Yong Yoo
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Korean J Pathol. 1990;24(1):1-9.
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- We have investigated an immunohistochemical expression of the Human-Ha-ras oncogene product p21 in tumor cells of the primary mass and metastatic lymph nodes with different histological features of gastric cancer by using avidinbiotin complex immunoperoxidase method in formalin-fixed tissue sections from 73 cases of primary tumor mass and 23 cases of metastatic lymph node. Histologic type of the gastric cancer was classification. The results obtained were as follows: 1) Expression of Ha-ras p21 was consistantly increased in the well differentiated tubular adenocarcinoma as compared with poorly differentiated tubular adenocarcinoma (p<0.01), and was substantially decreased in mucinous carcinoma and signet ring cell carcinoma. 2) Signet ring cell carcinoma showed that positive immunoperoxidase reaction for Ha-ras p21 exhibited in the majority of immature signet ring cell with scant cytoplasm rather than in the mature signet ring cells which have abundant cytoplasm filled with mucin. This findings indicate that mucin production from the tumor cell was not correlated with activation of ras gene in the tumor tissue of gastric carcinoma. 3) In general Ha-ras p21 expression was enhanced in the metastatic tumor cells of the regional lymph node compared with primary tumor, especially it was consistantly increase in the well differentiated tubular adenocarcinoma.
- The Effect of Uremic Plasma on the Proliferative Activity of CFU-GM in in-vitro Culture of Mouse Bone Marrow.
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Chang Soo Park, Joo Yong Yoo
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Korean J Pathol. 1987;21(4):215-226.
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- The increased susceptibility in patients of chronic renal failure to infection has been reported to be attributed to defects in granulocyte and lymphocyte function and proliferative activity of hematopoietic cells. The definite cause of the frequent infection in uremic patients, however, is still controversial. The effect of uremic plasma on the aspect of the hematopoietic cells has been scarcely been studied. In the present study, mouse bone marrow was cultured with uremic plasma, to evaluate the effect of uremic plasma on the proliferative activity and morphological features of CFU-GM. The results obtained were as follows. 1) The number of colonies in group co-cultured with uremic plasma was more reduced than that of normal plasma group. 2) There was no difference between the group cultured with predialytic uremic plasma and that of postdialytic plasma in number of colonies, macroclusters and microclusters. 3) The forms of colony were granulocytic and monocytic forms at 5 day of culture. Electron microscopically, granulocytes disclosed electron dense azurophilic granules and electrolucent specific granules in the cytoplasm, and monocyte showed numerous vesicles and vacuoles in the cytoplasm which had finger-like projections.
4) The molecular weight of inhibitory factor in the uremic plasma was supposed to be less than 50,000 daltons.
- Histochemical Muscle Fiber Types of Autopsied Human Gastrocnemius, Soleus, Peroneus longus and Tibialis anterior Muscles.
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Jin Chul Yang, Joo Yong Yoo
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Korean J Pathol. 1986;20(4):413-426.
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- This study was designed for the evaluation of the mean proportions and range of individual difference of muscle fiber types in human Gastrocneminus, Soleus, Peroneus longus and Tibialis anterior muscles, respectively. Flash-frozen muscle sections obtained from 15 cadavers were stained for H&E, trichrome, PAS, regular & reversed myosin ATPase, succinic dehydrogenase (SDH), acid phosphatase (Ac-Pase) and alkaline phosphatase (Alk-Pase). The results obtained were as follows. 1) Differentiation of muscle fiber types was not evident in H&E and trichrome stained specimen. Three types of muscle fibers were, however, evident in PAS-stained sections according to the degree of positivity in observed skeletal muscles. 2) Two fiber types (Types I and II) were only differentiated and the predominant muscle fiber type was type I by regular myosin ATPase reaction in Gastrocnemius, Soleus, Peroneus longus and Tibialis anterior muscles. 3) Three muscle fiber types (Type I, IIa and IIb) were, however, differentiated and the predominant muscle fiber type was type IIa, contrary to patterns of regular myosin ATPase in skeletal muscles except for Soleus muscle by reversed myosin ATPase stains. Only two muscle fiber types (Type I and II) and predominant type I fibers were shown in Soleus muscle. 4) SDH stains showed three types of muscle fiber except Soleus muscle. The mean proportions(%) of type 1 fibers were 48.0% in Gastrocnemius, 62.3% in Soleus, 40.8% in Peroneus longus, and 39.8% in Tibialis anterior muscles, respectively. 5) The mean proportions (%) of muscle fiber types in human skeletal muscles were markedly different, according to individuals and various enzyme histochemical stains. 6) The size of muscle fibers was relatevely small in type I fibers compared with type IIb. 7) Evaluation for the combination patterns of various enzyme histochemical activities showed that the common muscle fiber types (Type I, IIa and IIb) were markedly reduced in the mean proportions and unusual rare muscle fibers predominant. This predominance of unusual muscle fiber types and marked discrepancy in muscle fiber types between regular and reversed myosin ATPase seemed to be ascribed to decreased muscle enzyme activities following postmortem period.
- Immunohistochemical Study of S-100 Protein in Salivary Gland Tumors.
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Hyun Yeung Kang, Dong Wook Ryang, Joo Yong Yoo
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Korean J Pathol. 1986;20(4):427-434.
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- An immunohistochemistry for S-100 protein by biotin avidin system technique was done to evaluate the existence and distribution pattern of S-100 protein positive cells in various obtained were as follows. 1) Positive immunostaining for S-100 protein was observed in myoepithelial cell, serous acinar cell and nervous bundle in normal salivary gland. 2) Strong immunoreactivity for S-100 protein was shown in plemorphic adenoma, which was localized not only in myoepithelial cord or sheets of epithelial portion but also in chondrocytes, stellate cells of myxoid stroma and in squamous keratin pearl of mesenchymal metaplastic foci. 3) The S-100 protein was demonstrated in the tumor cells of tubular adenoma, acinic cell tumors and in epidermoid area of mucoepidermoid tumors. 4) Immunoreactivity for S-100 protein, however, was not found in the tumor cells of adenoid cystic carcinoma and adenolymphoma except for stroma reticulum cells. 5) Intensity of positive reaction for S-100 protein varied from cell to cell: Some had intense immunoreactivity, whreas others were only weakly positive or completely negative, even in myoepithelial cell nest of the same pleomorphic adenoma.
- Immunohistochemical Studies on the Serotonin, Somatostatin and Gastrin-Positive Cells in the Gastric Adenocarcinoma.
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Chan Choi, Joo Yong Yoo
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Korean J Cytopathol. 1985;19(4):391-401.
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- In order to study the histogenesis of gastric carcinoma, and to find the correlationship between the gastric carcinoma and endocrine tumor of digestive tract, we performed immunoperoxidase staining for serotonin, somatostatin and gastrin respectively in the 34 cases of surgically resected gastric carcinoma. The results were as follows; 1) Endocrine cells were found in 26.5% (9/34) of the gastric adenocarcinoma, but in 58.6% (17/29) in the normal gastric mucosa near the cancer area. The occurrence of endocrine cells was less frequent in cancer than in the normal gastric mucosa. 2) Among the 15 cases of intestinal type adenocarcinoma, serotonin-positive cells were found in 2 cases, somatostatin-positive cells in 1 case and gastrin-positive cells in 1 case. Among 16 cases of diffuse type adenocarcinoma, gastrin-positive cells were found in 2 cases, and somatostatin-positive cells were found in 2 cases. 3) We concluded that, the endocrine cells are more frequently observed in the well differentiated gastric carcinoma than poorly differentiated carcinoma tissue. In summary, gastric carcinomas are supposed to arise from the totipotent stem cells of endoderm origin, and they have multidirectional property of differentiation into both endocrine and nonendocrine cells. Most of them differentiate into nonendocrine cells and only a few of them differentiate into endocrine cells.
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